16s Metagenomics
Characterize complex microbial communities through targeted 16S rDNA sequencing.
Highlights:
The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the Ion Torrent™ semiconductor sequencing workflow. The kit permits PCR amplification of hypervariable regions of the 16S rDNA gene from bacteria. The amplified fragments can then be sequenced using the Ion PGM™ Sequencing 400 Kit on the Ion PGM™ platform and analysed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software.
The kit includes 2 sets of primers that can be used to amplify the corresponding hypervariable regions of the 16S rDNA gene in bacteria: Primer set V2-4-8 and Primer set V3-6,7-9.
These comprehensive primer sets allow for accurate detection and identification of a broad range of bacteria down to the genus or species level. The primer sets are optimized to tolerate high levels of PCR inhibitors and amplify targets from complex samples such as environmental, food, tissue and other challenging samples.
The primer sets are provided as two pools. Each pool can be used individually or in combination to allow for flexibility in experimental set up. When combined with the Ion Xpress™ Barcode Adapters kits, multiple polymicrobial samples can be combined in a single sequencing workflow.
Sequences generated using the Ion 16S™ Metagenomics Kit can be directly analysed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software enabling a rapid and semi-quantitative assessment of complex microbial samples.