DNA Barcoding – Molecular Identification

DNA Barcoding – Molecular Identification

DNA Barcoding is a powerful technique that uses short, standardized genetic markers to identify species with high accuracy. At Suparna?Biosciences, we train students and researchers in both the wet?lab workflow and bioinformatics analysis of DNA barcoding.

Workflow Modules

• DNA Extraction & Purification – High?quality template preparation.

• PCR Amplification – Targeting universal barcoding genes (COI, rbcL, 16S rRNA).

• Gel Electrophoresis & Visualization – Verification of amplicons.

• Sanger Sequencing – Gold?standard sequencing for species identification.

• Bioinformatics Analysis – Sequence alignment, BLAST search, and database submission (e.g., BOLD, GenBank).

• Applications

• Species identification in biodiversity studies.

• Authentication of food, herbal, and pharmaceutical products.

• Environmental monitoring and microbial diversity assessment.

• Forensic and conservation genetics.